Aspergillus terreus

Aspergillus Working Group description

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Title of Working Group

Aspergillus terreus

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Name(s) of Coordinator(s)

Cornelia Lass-Flörl

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Objectives

  1. Explore the genetic diversity and population dynamics of A. terreus. Under this aim, we propose to:
  2. develop a comprehensive culture repository comprising both clinical and environmental isolates of A. terreus and other isolates in section Terrei,
    1. design a multilocus sequence typing scheme (MLST) for species identification in Section Terrei,
    2. using the repository and the MLST scheme, generate data on the genetic diversity and population dynamics of A. terreus,
    3. establish a new typing method based on the polymorphism of tandem repeats in A. terreus,
    4. recognize and validly publish new species.
  3. Understand the epidemiology of A. terreus by
  4. Developing a microsatellite marker panel for strain discrimination and use test this panel on several environmental and clinical isolates of A. terreus to understand the molecular epidemiology of this organism
  5. Elucidating the clinical epidemiology of A. terreus
  6. Investigate amphotericin B resistance in A. terreus
  7. Study immune response and virulence potential of A. terreus
  8. To set up animal models to establish in vivo and in vitro correlation
  9. To create an A. terreus proteome map
  10. Database

A web-based data base will be built up and made accessible for all participants for studies.

  1. Prepare a genomic bank for A. terreus which will be use for the identification and characterization of some putative virulence factors of the fungus (e.g. anti-oxidant systems, proteases, etc.).
  2. Exo-metabolomics on Aspergillus terreus and related species.

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Achievements of the Working Group in last year (250 words)

Initiation of “TerrNet -A Global Aspergillus terreus Surveillance Study

(An initiative of the ISHAM Aspergillus terreus working group and ECMM)”

Publications from our group regarding Aspergillus terreus:
Escribano P, Peláez T, Recio S, Bouza E, Guinea J (2012) Characterization of clinical strains of Aspergillus terreus complex: molecular identification and antifungal susceptibility to azoles and amphotericin B. Clin Microbiol Infect 18: E24-26.
Lewis RE, Albert NP, Liao G, Wang W, Prince RA, Kontoyiannis DP (2013) High-dose induction liposomal amphotericin B followed by de-escalation is effective inexperimental Aspergillus terreus pneumonia. J Antimicrob Chemother 68: 1148-1151.
Arendrup MC, Jensen RH, Grif K, SkovM, Pressler T, Johansen HK, Lass-Flörl C (2012) In vivo emergence of Aspergillus terreus with reduced azole susceptibility and a Cyp51aM217L alteration. J Infect Dis 206: 981-985.
Lass-Flörl C (2012) Aspergillus terreus: how inoculums size and host characteristics affect its virulence. J Infect Dis 205: 1192-1194.
Genovese G, Leitner S, Minicante SA, Lass-Flörl C (2013) The Mediterranean red alga Asparagopsis taxiformis has antifungal activity against Aspergillus species. Mycoses 2013, doi: 10.1111/myc.12065. [ahead of publication].
Blum G, Hörtnagl C, Jukic E, Erbeznik T, Pümpel T, Dietrich H, Nagl M, Speth C, Rambach G, Lass-Flörl C (2013) New insights into Amphotericin B resistance in Aspergillus terreus. Antimicrob Agents Chemother 57: 1583-1588.
Blum G, Kainzner B, Grif K, Dietrich H, Zelger B, Sonnweber T, Lass-Flörl C (2013) In vitro and in vivo role of heat shock protein 90 in Amphotericin B resistance of Aspergillus terreus. Clin Microbiol Infect 19: 50-55.
Blum G, Kainzner B, Hörtnagl C, Nagl M, Speth C, Binder U, Maurer E, Sarg B, Lindner H, Gruber P, and Lass-Flörl C (2013) Heat shock protein 70, a cross-road for Amphotericin B resistance in Aspergillus terreus. Submitted for publication in The Journal of Infectious Diseases.
Speth C, Blum G; Hagleitner M, Hörtnagl C, Pfaller K, Posch B, Ott H, Würzner R, Lass-Flörl C, Rambach G (2013) Virulence and thrombocyte affectation of two Aspergillus terreus isolates differing in Amphotericin B susceptibility. Accepted for Publication in Medical Microbiology and Immunology.

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Is your Working Group going to continue for the next three years?

yes