Genotyping of Cryptococcous neoformans and C. gattii


Dra. Carolina Firacative

School of Medicine and Health Sciences, Universidad del Rosario

Bogota, Colombia






The objectives of the working group are:

1. to standardize genotyping within the C. neoformans/C. gattii species complex;

2. to establish the global molecular epidemiology of this species complex; finding biological characteristics that can represent the differences among genotypes.

Working Group participants


  • A congress session for the members of the working group took place as part of the 19th International Society for Human and Animal Mycology (ISHAM) Congress, in Melbourne, Australia, 2015. The participants discussed the current and future taxonomy of Cryptococcus neoformans/C. gattii and presented the molecular epidemiology data from different continents.
  • Establishment of The Latin American Network of Cryptococcosis involving researchers from Mexico, Costa Rica, Venezuela, Colombia, Argentina, Brazil and Peru.
  • Improvement of the MLST Database: 48 new sequence types of C. gattii and 166 new sequence types of C. neoformans were added to the database (See


Data was published from the European environmental survey:
Environmental distribution of Cryptococcus neoformans and C. gattii around the Mediterranean basin. FEMS Yeast Res. 2016 Jun;16(4) fow045. (Link here to the article)

Other published scientific papers which used the MLST database for C. neoformans and C. gattii:

Cogliati et al. Fungal Genet Biol. 2016;87:22-9 (Link here to the article)
Smith et al. Int J Med Microbiol. 2015;305(7):719-23 (Link here to the article)
Beale et al. PLoS Negl Trop Dis. 2015, 25;9(6):e0003847 (Link here to the article)
Desnos-Ollivier et al. MBio. 2015, 6(3):e00311 (Link here to the article)
Brito-Santos et al. PLoS One. 2015, 17;10(2):e0115866 (Link here to the article)
Linares et al. Rev Iberoam Micol. 2015;32(4):269-72 (Link here to the article)
Maestrale et al. Vet Microbiol. 2015,12;177(3-4):409-13 (Link here to the article)
Wu et al. Mycoses. 2015;58(5):280-7 (Link here to the article)
Dou et al. Eur J Clin Microbiol Infect Dis. 2015;34(4):753- 62 (Link here to the article)